Haptoglobin Mouse Monoclonal Antibody [5-D3]
cat.: M1510-37
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | 5-D3 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG1 |
| Immunogen: | Native Human Haptoglobin protein. |
| Positive control: | Human lung tissue lysate, human liver tissue lysate, HepG2, Hela, rat liver tissue, mouse liver tissue, human liver tissue, human lung cancer tissue. |
| Subcellular location: | Extracellular region, secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2,000 1:100-1:500 1:50-1:200 |
| Uniprot #: | SwissProt: P00738 Human |
| Alternative names: | Binding peptide BP Haptoglobin alpha chain Haptoglobin alpha(1S) beta Haptoglobin alpha(2FS) beta Haptoglobin beta chain Haptoglobin, alpha polypeptide Haptoglobin, beta polypeptide HP HP2 ALPHA2 HP2ALPHA2 HPA1S HPT HPT_HUMAN MGC111141 Zonulin |
Images
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Fig1: ICC staining Haptoglobin (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining Haptoglobin (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Haptoglobin antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Haptoglobin antibody. Counter stained with |
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Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Haptoglobin antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Haptoglobin antibody. Counter stained with hematoxylin. |
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Fig7:
Western blot analysis of Haptoglobin on Human plasma lysate with Rabbit anti-Haptoglobin antibody (M1510-37) at 1/4,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M1510-37, 1/4,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 45.2 kDa Observed band size: 45 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.