human IgM Mouse Monoclonal Antibody [B7-3]
cat.: M1510-5
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, ELISA |
| Clonality: | Monoclonal |
| Clone number: | B7-3 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 50 KD |
| Isotype: | IgG1 |
| Immunogen: | native protein. |
| Positive control: | Recombinant proteint lysates, human liver tissue, mouse spleen tissue. |
| Subcellular location: | Cell membrane, Immunoglobulin, Membrane, Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:200-1:500 1:50-1:100 |
| Uniprot #: | SwissProt: P01871 Human |
| Alternative names: | AGM1 Constant region of heavy chain of IgM DKFZp686I15196 DKFZp686I15212 FLJ00385 Hepatitis B virus receptor binding protein Ig mu chain C region IGHM IgM heavy chain constant region Immunoglobulin heavy constant mu Immunoglobulin mu chain Imunoglobulin heavy chain Imunoglobulin heavy chain constant region mu Imunoglobulin heavy chain mu constant region MGC104996 MGC52291 MU VH |
Images
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Fig1: Western blot analysis of human IgM on recombinant proteint lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1510-5, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-human IgM antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-5, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-human IgM antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-5, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.