PSD95 Mouse Monoclonal Antibody [B5-E6]
cat.: M1511-4
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | B5-E6 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 80 kDa |
| Isotype: | IgG2b |
| Immunogen: | Synthetic peptide within N-terminal human PSD95. |
| Positive control: | A172 cell lysate, N2A cell lysate, Neuro-2a, human brain tissue, Hela. |
| Subcellular location: | Cell membrane, postsynaptic density, synapse, Cytoplasm, axon, dendritic spine, dendrite, presynapse. |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000-1:5,000 1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P78352 Human | Q62108 Mouse | P31016 Rat |
| Alternative names: | Discs large homolog 4 Disks large homolog 4 DLG 4 Dlg4 DLG4_HUMAN FLJ97752 FLJ98574 Human post synaptic density protein 95 Post synaptic density protein 95 Postsynaptic density protein 95 PSD 95 PSD-95 PSD95 SAP 90 SAP-90 SAP90 Synapse associated protein 90 Synapse-associated protein 90 Tax interaction protein 15 |
Images
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Fig1:
Western blot analysis of PSD95 on different lysates with Mouse anti-PSD95 antibody (M1511-4) at 1/2,000 dilution. Lane 1: N2A cell lysate Lane 2: A172 cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 120 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M1511-4, 1/2,000 in 5% BSA at room temperature for 2 hours Secondary antibody: Goat Anti-Mouse IgG - HRP (HA1006), 1/5,000 , 1 hour at room temperature Predicted band size: 80.5 kDa Observed band size: 80 kDa |
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Fig2:
Immunocytochemistry analysis of Neuro-2a cells labeling PSD95 with Mouse anti-PSD95 antibody (M1511-4) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PSD95 antibody (M1511-4) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig3: Flow cytometric analysis of PSD95 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (M1511-4, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.