Anti-PSD95 antibody [B5-E6]
cat.: M1511-4
Product Type: Mouse monoclonal IgG2b, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Monoclonal
Clone number: B5-E6
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 80 kDa
Isotype: IgG2b
Immunogen: Synthetic peptide within N-terminal human PSD95.
Positive control: SHG-44 cell lysate, A172 cell lysate, N2A cell lysate, N2A, rat brain tissue, mouse brain tissue, Hela.
Subcellular location: Cell membrane, postsynaptic density, synapse, Cytoplasm, axon, dendritic spine, dendrite, presynapse.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC
1:2,000-1:5,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P78352 Human | Q62108 Mouse | P31016 Rat
Alternative names: Discs large homolog 4 Disks large homolog 4 DLG 4 Dlg4 DLG4_HUMAN FLJ97752 FLJ98574 Human post synaptic density protein 95 Post synaptic density protein 95 Postsynaptic density protein 95 PSD 95 PSD-95 PSD95 SAP 90 SAP-90 SAP90 Synapse associated protein 90 Synapse-associated protein 90 Tax interaction protein 15
Images
M1511-4_1.jpg Fig1: Western blot analysis of PSD95 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1511-4, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SHG-44 cell lysate
Lane 2: A172 cell lysate
Lane 3: N2A cell lysate
M1511-4_2.jpg Fig2: ICC staining of PSD95 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1511-4, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
M1511-4_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PSD95 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1511-4, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
M1511-4_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PSD95 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1511-4, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
M1511-4_5.jpg Fig5: Flow cytometric analysis of PSD95 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (M1511-4, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.