Cyclin A2 Mouse Monoclonal Antibody [N0-E3]
cat.: M1511-5
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P
Clonality: Monoclonal
Clone number: N0-E3
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 48 kDa
Isotype: IgG1
Immunogen: Recombinant protein within human p53 aa 50-432.
Positive control: 293 cell lysates, F9 cell lysates, PC12 cell lysates, Hela cell lysates, human tonsil tissue, human spleen tissue, human uterus tissue.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  ICC
  IHC-P

1:500
1:200
1:200
Uniprot #: SwissProt: P20248 Human | P51943 Mouse
Unigene: 13094 Rat
Alternative names: CCN1 CCNA Ccna2 CCNA2_HUMAN Cyclin A2 Cyclin-A Cyclin-A2
Images
M1511-5_1.jpg Fig1: Western blot analysis on different cell lysates using anti-Cyclin A2 Mouse mAb.
Positive control:
Lane 1: 293
Lane 2: F9
Lane 3: PC12
Lane 4: Hela
M1511-5_2.jpg Fig2: ICC staining Cyclin A2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
M1511-5_3.jpg Fig3: ICC staining Cyclin A2 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
M1511-5_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin.
M1511-5_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin.
M1511-5_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin.
M1511-5_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin.
M1511-5_8.jpg Fig8: Flow cytometric analysis of Hela cells with Cyclin A2 antibody at 1/100 dilution (black) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.