Cyclin A2 Mouse Monoclonal Antibody [N0-E3]
cat.: M1511-5
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | N0-E3 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human p53 aa 50-432. |
| Positive control: | 293 cell lysates, F9 cell lysates, PC12 cell lysates, Hela cell lysates, human tonsil tissue, human spleen tissue, human uterus tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:200 1:200 |
| Uniprot #: | SwissProt: P20248 Human | P51943 Mouse Unigene: 13094 Rat |
| Alternative names: | CCN1 CCNA Ccna2 CCNA2_HUMAN Cyclin A2 Cyclin-A Cyclin-A2 |
Images
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Fig1:
Western blot analysis on different cell lysates using anti-Cyclin A2 Mouse mAb. Positive control: Lane 1: 293 Lane 2: F9 Lane 3: PC12 Lane 4: Hela |
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Fig2: ICC staining Cyclin A2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Cyclin A2 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of Hela cells with Cyclin A2 antibody at 1/100 dilution (black) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.