Cyclin A2 Mouse Monoclonal Antibody [N0-E3]
cat.: M1511-5
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | N0-E3 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human p53 aa 50-432. |
| Positive control: | HEK-293 cell lysates, K-562 cell lysates, human tonsil tissue, human spleen tissue, human uterus tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:200 1:200 |
| Uniprot #: | SwissProt: P20248 Human | P51943 Mouse Unigene: 13094 Rat |
| Alternative names: | CCN1 CCNA Ccna2 CCNA2_HUMAN Cyclin A2 Cyclin-A Cyclin-A2 |
Images
|
Fig1:
Western blot analysis of Cyclin A2 on different lysates with Rabbit anti-Cyclin A2 antibody (M1511-5) at 1/5,000 dilution. Lane 1: HEK-293 (Human embryonic kidney cell) cell lysate Lane 2: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 180 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M1511-5, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 48.6 kDa Observed band size: 50 kDa |
|
Fig2: ICC staining Cyclin A2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: ICC staining Cyclin A2 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin. |
|
Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin. |
|
Fig7: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.