ICAM-1 Mouse Monoclonal Antibody [J0-F7]
cat.: M1511-6
| Product Type: | Mouse monoclonal IgG2c, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | J0-F7 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 58 kDa |
| Isotype: | IgG2c |
| Immunogen: | Recombinant protein within human ICAM-1 aa 30-300. |
| Positive control: | Raji cell lysates, Daudi cell lysates, Hela, HUVEC, Jurkat. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:2,000 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P05362 Human |
| Alternative names: | Antigen identified by monoclonal BB2 BB 2 BB2 CD 54 CD_antigen=CD54 CD54 Cell surface glycoprotein P3.58 Human rhinovirus receptor ICAM 1 ICAM-1 ICAM1 ICAM1_HUMAN intercellular adhesion molecule 1 (CD54), human rhinovirus receptor Intercellular adhesion molecule 1 Major group rhinovirus receptor MALA 2 MALA2 MyD 10 MyD10 P3.58 Surface antigen of activated B cells, BB2 |
Images
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Fig1: Western blot analysis of ICAM-1 on Raji cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1511-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of ICAM-1 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1511-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining of ICAM-1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1511-6, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining of ICAM-1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1511-6, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5: Flow cytometric analysis of ICAM-1 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (M1511-6, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a FITC-conjugated Goat anti-Mouse IgG Secondary antibody at 1/200 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.