PTP1B Mouse Monoclonal Antibody [D0-C7]
cat.: M1511-7
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | D0-C7 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human PTP1B aa 1-250. |
| Positive control: | A431 cell lysate, Hela cell lysate, A549 cell lysate, HepG2 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, C6 cell lysate, A431, human lung cancer tissue, mouse lung tissue. |
| Subcellular location: | Endoplasmic reticulum membrane |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:2,000 1:100-1:500 1:200-1:1,000 |
| Uniprot #: | SwissProt: P18031 Human | P35821 Mouse | P20417 Rat |
| Alternative names: | PTP1B Non receptor tyrosine phosphatase 1 Protein phosphotyrosylphosphatase 1B Protein tyrosine phosphatase 1B Protein tyrosine phosphatase non receptor type 1 Protein tyrosine phosphatase placental Protein-tyrosine phosphatase 1B PTN1_HUMAN PTP 1B PTP-1B PTPN 1 PTPN1 Tyrosine protein phosphatase non receptor type 1 Tyrosine-protein phosphatase non-receptor type 1 |
Images
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Fig1:
Western blot analysis of PTP1B on different lysates with Mouse anti-PTP1B antibody (M1511-7) at 1/500 dilution. Lane 1: A431 cell lysate Lane 2: Hela cell lysate Lane 3: A549 cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1511-7) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of PTP1B on different lysates with Rabbit anti-PTP1B antibody (M1511-7) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: PC-12 cell lysate Lane 3: C6 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1511-7) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of A431 cells labeling PTP1B with Mouse anti-PTP1B antibody (M1511-7) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PTP1B antibody (M1511-7) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Mouse anti-PTP1B antibody (M1511-7) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1511-7) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Mouse anti-PTP1B antibody (M1511-7) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1511-7) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.