PTP1B Mouse Monoclonal Antibody [D0-C7]
cat.: M1511-7
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | D0-C7 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human PTP1B aa 1-250. |
| Positive control: | A431 cell lysate, Hela cell lysate, A549 cell lysate, HepG2 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, C6 cell lysate, Hela, A549, HepG2, Jurkat. |
| Subcellular location: | Endoplasmic reticulum membrane |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000-1:2,000 1:100-1:500 1:50-1:100 |
| Uniprot #: | SwissProt: P18031 Human | P35821 Mouse | P20417 Rat |
| Alternative names: | PTP1B Non receptor tyrosine phosphatase 1 Protein phosphotyrosylphosphatase 1B Protein tyrosine phosphatase 1B Protein tyrosine phosphatase non receptor type 1 Protein tyrosine phosphatase placental Protein-tyrosine phosphatase 1B PTN1_HUMAN PTP 1B PTP-1B PTPN 1 PTPN1 Tyrosine protein phosphatase non receptor type 1 Tyrosine-protein phosphatase non-receptor type 1 |
Images
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Fig1:
Western blot analysis of PTP1B on different lysates with Mouse anti-PTP1B antibody (M1511-7) at 1/500 dilution. Lane 1: A431 cell lysate Lane 2: Hela cell lysate Lane 3: A549 cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1511-7) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of PTP1B on different lysates with Rabbit anti-PTP1B antibody (M1511-7) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: PC-12 cell lysate Lane 3: C6 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1511-7) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3: Immunocytochemical staining of Hela cells using anti-PTP1B Mouse mAb. |
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Fig4: Immunocytochemical staining of A549 cells using anti-PTP1B Mouse mAb. |
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Fig5: Immunocytochemical staining of HepG2 cells using anti-PTP1B Mouse mAb. |
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Fig6: Flow cytometric analysis of Jurkat cells with PTP1B antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.