Cytokeratin 8 Mouse Monoclonal Antibody [A1-B11]
cat.: M1603-2
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | A1-B11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 53 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within C-terminal human Cytokeratin 8 aa 441-483. |
| Positive control: | MCF-7, PANC-1, A549, PC12, human breast carcinoma tissue, human colon cancer tissue, mouse small intestine tissue, Hela. |
| Subcellular location: | Cytoplasm, Nucleus, Nucleus matrix. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000-1:2,000 1:100-1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P05787 Human | P11679 Mouse | Q10758 Rat |
| Alternative names: | CARD2 CK 8 CK-8 CK8 CYK8 CYKER Cytokeratin endo A Cytokeratin-8 DreK8 EndoA K2C8 K2C8_HUMAN K8 Keratin 8 Keratin type II cytoskeletal 8 Keratin, type II cytoskeletal 8 Keratin-8 KO Krt 2.8 KRT8 MGC118110 MGC174782 MGC53564 MGC85764 sb:cb186 Type-II keratin Kb8 |
Images
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Fig1:
Western blot analysis on different cell lysates using anti-Cytokeratin 8 Mouse mAb. Positive control: Lane 1: A549 Lane 2: MCF-7 Lane 3: PC12 |
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Fig2: Immunocytochemical staining of MCF-7 cells using anti-Cytokeratin 8 Mouse mAb. |
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Fig3: Immunocytochemical staining of PANC-1 cells using anti-Cytokeratin 8 Mouse mAb. |
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Fig4: Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-Cytokeratin 8 Mouse mAb. |
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Fig5: Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-Cytokeratin 8 Mouse mAb. |
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Fig6: Immunohistochemical analysis of paraffin- embedded mouse small intestine tissue using anti-Cytokeratin 8 Mouse mAb. |
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Fig7: Flow cytometric analysis of Hela cells with FGFR2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.