Cytokeratin 8 Mouse Monoclonal Antibody [A1-B11]
cat.: M1603-2
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | A1-B11 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 53 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within C-terminal human Cytokeratin 8 aa 441-483. |
| Positive control: | HeLa, A549, MCF7, PANC-1, human breast carcinoma tissue, human colon cancer tissue, mouse small intestine tissue, Hela. |
| Subcellular location: | Cytoplasm, Nucleus, Nucleus matrix. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000-1:2,000 1:100-1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P05787 Human | P11679 Mouse | Q10758 Rat |
| Alternative names: | CARD2 CK 8 CK-8 CK8 CYK8 CYKER Cytokeratin endo A Cytokeratin-8 DreK8 EndoA K2C8 K2C8_HUMAN K8 Keratin 8 Keratin type II cytoskeletal 8 Keratin, type II cytoskeletal 8 Keratin-8 KO Krt 2.8 KRT8 MGC118110 MGC174782 MGC53564 MGC85764 sb:cb186 Type-II keratin Kb8 |
Images
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Fig1:
Western blot analysis of Cytokeratin 8 on different lysates with Mouse anti-Cytokeratin 8 antibody (M1603-2) at 1/2,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cells）cell lysate Lane 2: A549 (Human lung adenocarcinoma cell) cell lysate Lane 3: MCF7 (Human breast cancer cell) cell lysate Lysates/proteins at 30 µg/Lane. Exposure time: 24 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M1603-2, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 53.7 kDa Observed band size: 40-54 kDa |
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Fig2: Immunocytochemical staining of MCF-7 cells using anti-Cytokeratin 8 Mouse mAb. |
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Fig3: Immunocytochemical staining of PANC-1 cells using anti-Cytokeratin 8 Mouse mAb. |
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Fig4: Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-Cytokeratin 8 Mouse mAb. |
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Fig5: Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-Cytokeratin 8 Mouse mAb. |
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Fig6: Immunohistochemical analysis of paraffin- embedded mouse small intestine tissue using anti-Cytokeratin 8 Mouse mAb. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.