Hsp90 alpha Mouse Monoclonal Antibody [A10-A5-D2]
cat.: M1603-3
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | A10-A5-D2 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 90kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human Hsp90 alpha aa 1-50 / 732. |
| Positive control: | Hela, PC12, NIH/3T3, SW480, Ags, human kidney tissue, human gastric carcinoma tissue, mouse testis tissue. |
| Subcellular location: | Nucleus, Cytoplasm, Melanosome, Cell membrane, Mitochondrion. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000-1:2,000 1:100-1:500 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P07900 Human |
| Alternative names: | EL52 epididymis luminal secretory protein 52 Heat shock 86 kDa heat shock 90kD protein 1, alpha Heat shock 90kD protein 1, alpha like 4 heat shock 90kD protein, alpha-like 4 Heat shock 90kDa protein 1 alpha Heat shock protein 90kDa alpha (cytosolic) class A member 1 Heat shock protein HSP 90-alpha HS90A_HUMAN HSP 86 HSP86 Hsp89 HSP89A Hsp90 HSP90A HSP90AA1 HSP90ALPHA HSP90N HSPC1 HSPCA HSPCAL1 HSPCAL4 HSPN LAP 2 LAP2 lipopolysaccharide-associated protein 2 LPS-associated protein 2 Renal carcinoma antigen NY-REN-38 |
Images
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Fig1:
Western blot analysis on different cell lysates using anti-Hsp90 alpha Mouse mAb. Positive control: Lane 1: Hela Lane 2: 293T Lane 3: PC12 Lane 4: NIH/3T3 |
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Fig2: Immunocytochemical staining of Hela cells using anti-Hsp90 alpha Mouse mAb. |
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Fig3: Immunocytochemical staining of SW480 cells using anti-Hsp90 alpha Mouse mAb. |
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Fig4: Immunocytochemical staining of Ags cells using anti-Hsp90 alpha Mouse mAb. |
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Fig5: Immunocytochemical staining of NIH/3T3 cells using anti-Hsp90 alpha Mouse mAb. |
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Fig6: Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-Hsp90 alpha Mouse mAb. |
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Fig7: Immunohistochemical analysis of paraffin- embedded human gastric carcinoma tissue using anti-Hsp90 alpha Mouse mAb. |
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Fig8: Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-Hsp90 alpha Mouse mAb. |
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Fig9: Flow cytometric analysis of Hela cells with Hsp90 alpha antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.