PP2A alpha + beta Mouse Monoclonal Antibody [A0-G7]
cat.: M1603-4
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A0-G7 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human PP2A alpha + beta aa 260-309 / 309. |
| Positive control: | A431 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, F9 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, Hela, MCF-7, SHG-44. |
| Subcellular location: | Cytoplasm, Nucleus, Chromosome, centromere, cytoskeleton, spindle pole. |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000-1:5,000 1:100-1:500 1:50-1:200 |
| Uniprot #: | SwissProt: P67775 Human | P62714 Human | P63330 Mouse | P62715 Mouse | P63331 Rat | P62716 Rat |
| Alternative names: | PP2A A PP2A alpha PP2A B PP2A beta PP2A-alpha PP2A-beta PP2AA_HUMAN PP2Aalpha PP2AB_HUMAN PP2Abeta PP2Ac PP2CA PP2Calpha PP2CB PPP2CA PPP2CB Protein phosphatase 2 catalytic subunit alpha isoform Protein phosphatase 2 catalytic subunit beta isoform Protein phosphatase type 2A catalytic subunit Replication protein C RP C RP-C RPC Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform Serine/threonine protein phosphatase 2A catalytic subunit beta isoform Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform |
Images
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Fig1:
Western blot analysis of PP2A alpha + beta on different lysates with Mouse anti-PP2A alpha + beta antibody (M1603-4) at 1/2,000 dilution. Lane 1: A431 cell lysate (10 µg/Lane) Lane 2: 293T cell lysate (10 µg/Lane) Lane 3: NIH/3T3 cell lysate (10 µg/Lane) Lane 4: F9 cell lysate (10 µg/Lane) Lane 5: PC-12 cell lysate (10 µg/Lane) Lane 6: Mouse brain tissue lysate (20 µg/Lane) Lane 7: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1603-4) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining PP2A(alpha+beta) (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining PP2A(alpha+beta) (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining PP2A(alpha+beta) (green) in SHG-44 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Flow cytometric analysis of Hela cells with PP2A(alpha+beta) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.