PP2A(alpha+beta) Mouse Monoclonal Antibody [A0-G7]
cat.: M1603-4
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A0-G7 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 35 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human PP2A(alpha+beta) aa 260-309 / 309. |
| Positive control: | A431 cell lysate, Hela, MCF-7, SHG-44. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000-1:5,000 1:100-1:500 1:50-1:200 |
| Uniprot #: | SwissProt: P62714 Human | P67775 Human |
| Alternative names: | PP2A A PP2A alpha PP2A B PP2A beta PP2A-alpha PP2AA_HUMAN PP2Ac PP2CB PPP2CA PPP2CB Protein phosphatase 2 catalytic subunit alpha isoform Protein phosphatase 2 catalytic subunit beta isoform Replication protein C RP C RP-C Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform Serine/threonine protein phosphatase 2A catalytic subunit beta isoform Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform |
Images
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Fig1: Western blot analysis of PP2A(alpha+beta) on A431 cell lysate using anti-PP2A(alpha+beta) antibody at 1/1000 dilution. |
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Fig2: ICC staining PP2A(alpha+beta) (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining PP2A(alpha+beta) (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining PP2A(alpha+beta) (green) in SHG-44 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Flow cytometric analysis of Hela cells with PP2A(alpha+beta) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.