LAMP2 Mouse Monoclonal Antibody [C9-9]
cat.: M1603-5
Product Type: Mouse monoclonal IgG2b, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Monoclonal
Clone number: C9-9
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: 45 kD(Predicted band size)
Isotype: IgG2b
Immunogen: Recombinant protein corresponding to N terminal Human LAMP2.
Positive control: Hela cell lysates, HeLa, HepG2, human liver tissue, human kidney tissue, human pancreas tissue.
Subcellular location: Cell membrane, Cytoplasmic vesicle, Endosome, Lysosome, Membrane.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC
1:500-1:2000
1:100-1:500
1:50-1:100
1:50-1:100
Uniprot #: SwissProt: P13473 Human
Alternative names: CD107 antigen like family member B CD107 antigen-like family member B CD107b LAMP 2 LAMP 2C LAMP-2 LAMP2 LAMP2_HUMAN LAMPB LGP 96 LGP110 LGP96 Lysosomal associated membrane protein 2 Lysosome associated membrane glycoprotein 2 Lysosome associated membrane protein 2 Lysosome-associated membrane glycoprotein 2 Lysosome-associated membrane protein 2 MAC3
Images
M1603-5_1.jpg Fig1: Western blot analysis of LAMP2 on Hela cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1603-5, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
M1603-5_2.jpg Fig2: Immunocytochemistry analysis of HeLa cells labeling LAMP2 with Mouse anti-LAMP2 antibody (M1603-5) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-LAMP2 antibody (M1603-5) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
M1603-5_3.jpg Fig3: ICC staining of LAMP2 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1603-5, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
M1603-5_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-LAMP2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1603-5, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
M1603-5_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LAMP2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1603-5, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
M1603-5_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-LAMP2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1603-5, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
M1603-5_7.jpg Fig7: Flow cytometric analysis of LAMP2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (M1603-5, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.