Product Type: | Mouse monoclonal IgG1, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | A5-C9-G6 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 72 kDa |
Isotype: | IgG1 |
Immunogen: | Synthetic peptide within Human GRP78 aa 1-50 / 654. |
Positive control: | L-929 cell lysate, U-87 MG cell lysate, RAW264.7 cell lysate, RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate, mouse liver tissue lysate, rat liver tissue lysate, rat pancreas tissue lysate. |
Subcellular location: | Endoplasmic reticulum lumen. Melanosome. Cytoplasm. |
Recommended Dilutions:
WB |
1:1,000-1:5,000 |
Uniprot #: | SwissProt: P11021 Human | P20029 Mouse | P06761 Rat |
Alternative names: | 78 kDa glucose regulated protein 78 kDa glucose-regulated protein AL022860 AU019543 BIP D2Wsu141e D2Wsu17e Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 Endoplasmic reticulum lumenal Ca2+ binding protein grp78 Epididymis secretory sperm binding protein Li 89n FLJ26106 Glucose Regulated Protein 78kDa GRP 78 GRP-78 GRP78 GRP78_HUMAN Heat shock 70 kDa protein 5 Heat Shock 70kDa Protein 5 Heat shock protein family A (Hsp70) member 5 HEL S 89n Hsce70 HSPA 5 HSPA5 Immunoglobulin Heavy Chain Binding Protein Immunoglobulin heavy chain-binding protein mBiP MIF2 Sez7 |
Fig1:
Western blot analysis of GRP78 / BIP on different lysates with Mouse anti-GRP78 / BIP antibody (M1701-1) at 1/1,000 dilution. Lane 1: L-929 cell lysate Lane 2: U-87 MG cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate Lane 5: Mouse liver tissue lysate Lane 6: Rat liver tissue lysate Lane 7: Rat pancreas tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 72 kDa Observed band size: 70/72 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1701-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |