GRP78 / BIP Mouse Monoclonal Antibody [A5-C9-G6]
cat.: M1701-1
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: A5-C9-G6
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 72 kDa
Isotype: IgG1
Immunogen: Synthetic peptide within Human GRP78 aa 1-50 / 654.
Positive control: L-929 cell lysate, U-87 MG cell lysate, RAW264.7 cell lysate, RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate, mouse liver tissue lysate, rat liver tissue lysate, rat pancreas tissue lysate.
Subcellular location: Endoplasmic reticulum lumen. Melanosome. Cytoplasm.
Recommended Dilutions:
  WB

1:1,000-1:5,000
Uniprot #: SwissProt: P11021 Human | P20029 Mouse | P06761 Rat
Alternative names: 78 kDa glucose regulated protein 78 kDa glucose-regulated protein AL022860 AU019543 BIP D2Wsu141e D2Wsu17e Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 Endoplasmic reticulum lumenal Ca2+ binding protein grp78 Epididymis secretory sperm binding protein Li 89n FLJ26106 Glucose Regulated Protein 78kDa GRP 78 GRP-78 GRP78 GRP78_HUMAN Heat shock 70 kDa protein 5 Heat Shock 70kDa Protein 5 Heat shock protein family A (Hsp70) member 5 HEL S 89n Hsce70 HSPA 5 HSPA5 Immunoglobulin Heavy Chain Binding Protein Immunoglobulin heavy chain-binding protein mBiP MIF2 Sez7
Images
M1701-1_1.jpg Fig1: Western blot analysis of GRP78 / BIP on different lysates with Mouse anti-GRP78 / BIP antibody (M1701-1) at 1/1,000 dilution.

Lane 1: L-929 cell lysate
Lane 2: U-87 MG cell lysate
Lane 3: RAW264.7 cell lysate
Lane 4: RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate
Lane 5: Mouse liver tissue lysate
Lane 6: Rat liver tissue lysate
Lane 7: Rat pancreas tissue lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 72 kDa
Observed band size: 70/72 kDa

Exposure time: 6 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1701-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.