| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | 4E1 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 10kDa |
| Isotype: | IgG2a |
| Immunogen: | Synthetic peptide within Human BLCAP aa 38-87 / 87. |
| Positive control: | HepG2, rat cervix tissue, human breast tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P62952 Human | P62950 Rat |
| Alternative names: | Bc10 Bladder cancer 10 kDa protein Bladder cancer related protein (10kD) Bladder cancer-associated protein BLCAP BLCAP_HUMAN |
|
Fig1: Western blot analysis of BLCAP on recombinant protein tissue lysate using anti-BLCAP antibody at 1/5,000 dilution. |
|
Fig2: ICC staining BLCAP (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-BLCAP antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat cervix tissue using anti-BLCAP antibody. Counter stained with hematoxylin. |