CD137 Mouse Monoclonal Antibody [2G1]
cat.: M1701-11
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | 2G1 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant full length protein. |
| Positive control: | K-562 (Human chronic myelogenous leukemia cell),A431, A549, LOVO, rat brain tissue, rat hippocampus tissue, human liver cancer tissue, mouse brain tissue, human tonsil tissue. |
| Subcellular location: | Membrane. Extracellular space. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1000 1:100-1:500 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q07011 Human | P20334 Mouse |
| Alternative names: | 4 1BB 4 1BB ligand receptor 4-1BB ligand receptor 4-1BB Ligand Receptor T Cell 4-1BB, mouse, homolog of Antigen 4-1BB Homolog CD 137 CD137 CD137 antigen CDw137 HLDA VI Homolog of mouse 4 1BB ILA induced by lymphocyte activation (ILA) Induced by lymphocyte activation Interleukin activated receptor homolog of mouse Ly63 Ly63, mouse, homolog of MGC2172 OTTHUMP00000044294 Receptor protein 4 1BB T cell antigen 4 1BB homolog T cell antigen ILA T-cell antigen 4-1BB homolog T-cell antigen ILA TNF receptor superfamily member 9 TNFRSF9 TNR9_HUMAN Tumor necrosis factor receptor superfamily member 9 |
Images
|
Fig1:
Western blot analysis of CD137 on different lysates with Rabbit anti-CD137 antibody (M1701-11) at 1/1,000 dilution. Lane 1: K-562 (Human chronic myelogenous leukemia cell) Lysates/proteins at 15 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M1701-11, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/100,000 in 5% NFDM/TBST, 1 hour at room temperature |
|
Fig2: ICC staining CD137 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: ICC staining CD137 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig4: ICC staining CD137 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CD137 antibody. Counter stained with hematoxylin. |
|
Fig6: Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue using anti-CD137 antibody. Counter stained with hematoxylin. |
|
Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD137 antibody. Counter stained with hematoxylin. |
|
Fig8: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CD137 antibody. Counter stained with hematoxylin. |
|
Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD137 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.