GRP94 Mouse Monoclonal Antibody [F1-D10-G3]
cat.: M1701-12
| Product Type: | Mouse monoclonal IgM, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | F1-D10-G3 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 94 kDa |
| Isotype: | IgM |
| Immunogen: | Recombinant protein within Human GRP94 aa 201-400 / 803. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, human brain tissue lysate, NIH/3T3 cell lysate, mouse placenta tissue lysate, mouse brain tissue lysate, PC-12 cell lysate, rat placenta tissue lysate, rat brain tissue lysate, NIH-3T3, human liver cancer tissue, human kidney tissue, mouse testis tissue, mouse brain tissue. |
| Subcellular location: | Endoplasmic reticulum lumen, melanosome. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:10,000 1:50 1:50-1:200 |
| Uniprot #: | SwissProt: P14625 Human | P08113 Mouse | Q66HD0 Rat |
| Alternative names: | 94 kDa glucose regulated protein 94 kDa glucose-regulated protein ECGP Endoplasmin Endothelial cell (HBMEC) glycoprotein ENPL_HUMAN Glucose regulated protein 94kDa gp96 gp96 homolog GRP 94 GRP-94 Heat shock protein 90 kDa beta member 1 heat shock protein 90kDa beta (Grp94), member 1 Heat shock protein, 90 kDa, beta, 1 HSP90B1 Stress inducible tumor rejection antigen GP96 TRA1 tumor rejection antigen (gp96) 1 Tumor rejection antigen 1 Tumor rejection antigen gp96 Tumor rejection antigen-1 (gp96) |
Images
|
Fig1:
Western blot analysis of GRP94 on different lysates with Mouse anti-GRP94 antibody (M1701-12) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (10 µg/Lane) Lane 2: HeLa cell lysate (10 µg/Lane) Lane 3: Human brain tissue lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (10 µg/Lane) Lane 5: Mouse placenta tissue lysate (20 µg/Lane) Lane 6: Mouse brain tissue lysate (20 µg/Lane) Lane 7: PC-12 cell lysate (10 µg/Lane) Lane 8: Rat placenta tissue lysate (20 µg/Lane) Lane 9: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 92 kDa Observed band size: 94 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1701-12) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: ICC staining GRP94 (green) in NIH-3T3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-GRP94 antibody (M1701-12) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1701-12) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GRP94 antibody. Counter stained with hematoxylin. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-GRP94 antibody. Counter stained with hematoxylin. |
|
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GRP94 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.