gamma tubulin Mouse Monoclonal Antibody [10-A4]
cat.: M1701-13
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | 10-A4 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa |
| Isotype: | IgG2a |
| Immunogen: | Synthetic peptide within Human gamma tubulin aa 402-451 / 451. |
| Positive control: | Rat epididymis tissue, human tonsil tissue, human colon cancer tissue, mouse testis tissue. |
| Subcellular location: | Cytoplasm, cytoskeleton, centrosome. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:5,000 1:100-1:500 1:50-1:200 |
| Uniprot #: | SwissProt: P23258 Human | P83887 Mouse | P83888 Rat |
| Alternative names: | Gamma 1 tubulin Gamma 2 tubulin Gamma Tubulin 1 Gamma Tubulin 2 Gamma tubulin complex component 1 Gamma-2-tubulin GCP 1 GCP1 MGC131994 TBG2_HUMAN TUBG TUBG1 TUBG2 TUBGCP1 Tubulin gamma 1 chain Tubulin gamma 2 chain Tubulin gamma complex-associated protein 1 Tubulin gamma-2 chain tubulin, gamma 1 tubulin, gamma 2 tubulin, gamma polypeptide Xgam |
Images
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Fig1:
Western blot analysis of gamma tubulin on different lysates using anti-gamma tubulin antibody at 1/2,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela Lane 3: Human fetal brain tissue |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-gamma tubulin antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-gamma tubulin antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-gamma tubulin antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-gamma tubulin antibody. Counter stained with hematoxylin. |
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Fig6:
Immunocytochemistry analysis of Hela cells labeling gamma tubulin with Mouse anti-gamma tubulin antibody (M1701-13) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at RT, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-gamma tubulin antibody (M1701-13) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.