Tyrosinase Mouse Monoclonal Antibody [C2-B5]
cat.: M1701-2
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | C2-B5 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human Tyrosinase aa 51-100 / 529. |
| Positive control: | Melanoma cells lysates, B16F1, human urethral melanoma tissue. |
| Subcellular location: | Melanosome membrane, Melanosome. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P14679 Human | Q2TA50 Mouse |
| Alternative names: | ATN CMM8 LB24 AB LB24-AB Monophenol monooxygenase OCA1 OCA1A OCAIA Oculocutaneous albinism IA SHEP3 SK29 AB SK29-AB Tumor rejection antigen AB TYR TYRO_HUMAN tyrosinase (oculocutaneous albinism IA) Tyrosinase |
Images
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Fig1: Western blot analysis of tyrosinase on melanoma cells lysates using anti- tyrosinase antibody at 1/1,000 dilution. |
|
Fig2: ICC staining tyrosinase in B16F1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human urethral melanoma tissue using anti- tyrosinase antibody. Counter stained with hematoxylin. |
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Fig4: Flow cytometric analysis of B16F1 cells with tyrosinase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.