MAL Mouse Monoclonal Antibody [B5-G3]
cat.: M1701-6
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | B5-G3 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within mouse MAL aa 97-146 / 153. |
| Positive control: | Mouse kidney tissue, mouse lymphatic vessels tissue lysate, rat brain tissue, rat kidney tissue, mouse brain tissue, Jurkat. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:50-1:600 1:50-1:200 |
| Uniprot #: | SwissProt: P21145 Human | O09198 Mouse | Q64349 Rat |
| Alternative names: | mal MAL protein gene Mal T-cell differentiation protein MAL_HUMAN MALGENE MPV17 Myelin and lymphocyte protein T-cell differentiation protein MAL T-lymphocyte maturation-associated protein VIP17 |
Images
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Fig1: Western blot analysis of MAL on mouse kidney (1) and mouse lymphatic vessels (2) tissue lysate using anti-MAL antibody at 1/1,000 dilution. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-MAL antibody (M1701-6) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1701-6) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-MAL antibody (M1701-6) at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1701-6) at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-MAL antibody (M1701-6) at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1701-6) at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5: Flow cytometric analysis of Jurkat cells with MAL antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.