MAL Mouse Monoclonal Antibody [B5-G3]
cat.: M1701-6
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: B5-G3
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 17 kDa
Isotype: IgG1
Immunogen: Synthetic peptide within mouse MAL aa 97-146 / 153.
Positive control: Mouse kidney tissue, mouse lymphatic vessels tissue lysate, rat brain tissue, rat kidney tissue, mouse brain tissue, Jurkat.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500-1:1,000
1:50-1:600
1:50-1:200
Uniprot #: SwissProt: P21145 Human | O09198 Mouse | Q64349 Rat
Alternative names: mal MAL protein gene Mal T-cell differentiation protein MAL_HUMAN MALGENE MPV17 Myelin and lymphocyte protein T-cell differentiation protein MAL T-lymphocyte maturation-associated protein VIP17
Images
M1701-6_1.jpg Fig1: Western blot analysis of MAL on mouse kidney (1) and mouse lymphatic vessels (2) tissue lysate using anti-MAL antibody at 1/1,000 dilution.
M1701-6_2.png Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-MAL antibody (M1701-6) at 1/600 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1701-6) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
M1701-6_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-MAL antibody. Counter stained with hematoxylin.
M1701-6_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MAL antibody. Counter stained with hematoxylin.
M1701-6_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-MAL antibody. Counter stained with hematoxylin.
M1701-6_6.jpg Fig6: Flow cytometric analysis of Jurkat cells with MAL antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.