GPX5 Mouse Monoclonal Antibody [5A1]
cat.: M1701-8
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | 5A1 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human GPX5 aa 85-134 / 221. |
| Positive control: | 293T cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, U-937. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000-1:5,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: O75715 Human | P21765 Mouse | P30710 Rat |
| Alternative names: | Arep arMEP24 EGLP Epididymal androgen related protein Epididymal secretory glutathione peroxidase epididymis secretory sperm binding protein Li 75p Epididymis specific glutathione peroxidase like protein Epididymis-specific glutathione peroxidase-like protein Glutathione peroxidase 5 Glutathione peroxidase 5 (epididymal androgen related protein) Glutathione peroxidase, epididymal GPx-5 GPX5 GPX5_HUMAN GSHPx-5 HEL-S-75p Major androgen regulated protein OTTHUMP00000017919 OTTHUMP00000214636 |
Images
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Fig1:
Western blot analysis of GPX5 on different lysates with Mouse anti-GPX5 antibody (M1701-8) at 1/5,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane) Lane 2: Mouse testis tissue lysate (40 µg/Lane) Lane 3: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 25 kDa Observed band size: 20 kDa Exposure time: Lane 1: 3 minutes; Lane 2-3: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1701-8) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of U-937 cells labeling GPX5 with Mouse anti-GPX5 antibody (M1701-8) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-GPX5 antibody (M1701-8) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Flow cytometric analysis of U-937 cells labeling GPX5. Cells were fixed and permeabilized. Then stained with the primary antibody (M1701-8, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.