GPX5 Mouse Monoclonal Antibody [5A1]
cat.: M1701-8
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | 5A1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human GPX5 aa 85-134 / 221. |
| Positive control: | Human sperm tissue lysates, mouse testis tissue lysates, 293T, Hela, human liver tissue, mouse liver tissue, mouse testis tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500-1:5,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: O75715 Human | P21765 Mouse |
| Alternative names: | Arep arMEP24 EGLP Epididymal androgen related protein Epididymal secretory glutathione peroxidase epididymis secretory sperm binding protein Li 75p Epididymis specific glutathione peroxidase like protein Epididymis-specific glutathione peroxidase-like protein Glutathione peroxidase 5 Glutathione peroxidase 5 (epididymal androgen related protein) Glutathione peroxidase, epididymal GPx-5 GPX5 GPX5_HUMAN GSHPx-5 HEL-S-75p Major androgen regulated protein OTTHUMP00000017919 OTTHUMP00000214636 |
Images
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Fig1: Western blot analysis of GPX5 on human sperm tissue lysate using anti-GPX5 antibody at 1/5,000 dilution. |
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Fig2: Western blot analysis of GPX5 on mouse testis tissue lysate using anti-GPX5 antibody at 1/500 dilution. |
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Fig3: ICC staining GPX5 (green) in 293T cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining GPX5 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GPX5 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-GPX5 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-GPX5 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.