Cytochrome C Mouse Monoclonal Antibody [10-E11-G2]
cat.: M1701-9
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | 10-E11-G2 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 12 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within human Cytochrome C aa 2-60. |
| Positive control: | Mouse brain tissue lysate, Mouse kidney tissue lysate, Hela, HepG2, MCF-7, human liver tissue, human spleen tissue, human kidney tissue, mouse heart tissue. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:200-1:500 1:50-1:200 1:50-1:600 |
| Uniprot #: | SwissProt: P99999 Human | P62897 Mouse | P62898 Rat |
| Alternative names: | CYC CYC_HUMAN CYCS Cytochrome c Cytochrome c somatic HCS THC4 |
Images
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Fig1:
Western blot analysis of Cytochrome C on different lysates with Mouse anti-Cytochrome C antibody (M1701-9) at 1/500 dilution. Lane 1: Mouse brain tissue lysate(20 µg/Lane) Lane 2: Mouse kidney tissue lysate (20 µg/Lane) Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time: 30 seconds; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1701-9) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining Cytochrome C (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Cytochrome C (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Cytochrome C (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytochrome C antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Cytochrome C antibody. Counter stained with hematoxylin. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-Cytochrome C antibody (M1701-9) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1701-9) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Cytochrome C antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.