HRP Conjugated Anti-mouse IgG for IP Nano-secondary antibody
cat.: NBI02H
| Product Type: | Alpaca monoclonal IgG, nano secondary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Form: | Liquid |
| Storage condition: | Store at –20 ℃, protect from light. |
| Storage buffer: | PBS PH=7.5, 10mg/ml BSA, 100mM trehalose, 50% glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Recombinant Expression and Affinity purified,Dissociation constant KD of 0.1nM. |
| Isotype: | IgG |
| Immunogen: | Mouse IgG |
| Recommended Dilutions:
WB IP |
1:1,000-1:10,000 sample should be completely reduced/denatured before loaded onto a western blot. |
Images
|
Fig1:
Immunoprecipitating IgG and DYKDDDDK Tag (FLAG)(M1403-2) in recombinant protein with falg on N-terminal. 25ng of Immunoprecipitated protein incubated with primary antibody (1/1000) for over night at 4℃. For western blotting a IP Detection Reagent (NBI02H) (1/1000) was used to confirm successful immunoprecipation. Lane1/2: Immunoprecipitating IgG in recombinant protein with flag on N-terminal Lane3: Immunoprecipitating DYKDDDDK Tag (FLAG) (M1403-2) in recombinant protein with flag on N-terminal |
|
Fig2:
PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with HA601172 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA601172 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA601172 IP in HeLa cell lysate Lane 3: Mouse IgG instead of HA601172 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds; ECL: K1801 |
|
Fig3:
PCNA was immunoprecipitated from 0.2 mg NIH/3T3 cell lysate with HA601172 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA601172 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 cell lysate (input) Lane 2: HA601172 IP in NIH/3T3 cell lysate Lane 3: Mouse IgG instead of HA601172 in NIH/3T3 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds; ECL: K1802 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.