beta Actin Rabbit Polyclonal Antibody
cat.: R1102-1
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 42 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal residues of Human beta actin.
Positive control: Different cell lysates, MCF-7, human kidney tissue, human tonsil tissue, human colon cancer tissue, HT-29.
Subcellular location: Cytoskeleton, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:1,000-2,000
1:50-1:200
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P60709 Human | P60710 Mouse | P60711 Rat
Alternative names: A26C1A A26C1B ACTB ACTB_HUMAN Actin beta Actin cytoplasmic 1 Actin, cytoplasmic 1, N-terminally processed Actx b actin Beta cytoskeletal actin Beta-actin BRWS1 E430023M04Rik MGC128179 PS1TP5 binding protein 1 PS1TP5BP1 β-actin β actin
Images
R1102-1_1.jpg Fig1: Western blot analysis of β-Actin on different cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:50,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane A: PC12 cell lysates
Lane B: Hela cell lysates
Lane C: NIH/3T3 cell lysates
R1102-1_2.jpg Fig2: ICC staining of β-Actin in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1102-1, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
R1102-1_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-β-Actin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1102-1, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
R1102-1_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-β-Actin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1102-1, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
R1102-1_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-β-Actin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1102-1, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
R1102-1_6.jpg Fig6: Flow cytometric analysis of β-Actin was done on HT-29 cells. The cells were fixed, permeabilized and stained with the primary antibody (R1102-1, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.