Autoimmune regulator (AIRE) Rabbit Polyclonal Antibody
cat.: R1107-1
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human AIRE. |
| Positive control: | Transfected 293T cell lysate, Human thymus tissue, human testis tissue. |
| Subcellular location: | Nucleus, Cytoplasm |
| Recommended Dilutions:
WB IHC-P |
1:500- 1:1,000 1:50 |
| Uniprot #: | SwissProt: O43918 Human |
| Alternative names: | AIRE AIRE_HUMAN AIRE1 APECED APECED protein APS1 APSI Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein Autoimmune regulator Autoimmune regulator protein PGA1 |
Images
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Fig1: Western blot-AIRE antibody A: 293T cell lysate, transfected with human AIRE B: 293T cell lysate, untransfected |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human thymus tissue with Rabbit anti-Autoimmune regulator (AIRE) antibody (R1107-1) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1107-1) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Autoimmune regulator (AIRE) antibody (R1107-1) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1107-1) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.