CD14 Rabbit Polyclonal Antibody
cat.: R1107-2
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 40kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CD14 aa 154-196. |
| Positive control: | K562, SW480, Raji |
| Subcellular location: | Cell membrane, Golgi apparatus , Secreted. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:50 1:50 |
| Uniprot #: | SwissProt: P08571 Human | P10810 Mouse |
| Alternative names: | CD 14 CD_antigen=CD14 CD14 CD14 antigen CD14 molecule CD14_HUMAN LPS-R Mo2 Monocyte differentiation antigen CD14 Monocyte differentiation antigen CD14 urinary form Monocyte differentiation antigen CD14, membrane-bound form Myeloid cell specific leucine rich glycoprotein Myeloid cell-specific leucine-rich glycoprotein |
Images
|
Fig1: Western blot analysis on K562 cell lysates using anti-CD14 rabbit polyclonal antibody. |
|
Fig2: ICC staining CD14 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: Flow cytometric analysis of Raji cells with CD14 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.