FOXD3 Rabbit Polyclonal Antibody
cat.: R1107-4
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to of Mouse FOXD3 aa 1-50 / 465. |
| Positive control: | NCCIT cell lysate, HEK-293 cell lysate, RAW264.7 cell lysate |
| Subcellular location: | Nucleus |
| Recommended Dilutions:
WB |
1:1,000-1:5,000 |
| Uniprot #: | SwissProt: Q9UJU5 Human | Q61060 Mouse |
| Alternative names: | AIS1 forkhead box D3 Forkhead box protein D3 foxd3 FOXD3_HUMAN Genesis HFH2 HNF3/FH transcription factor genesis VAMAS2 |
Images
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Fig1:
Western blot analysis of FOXD3 on different lysates with Rabbit anti-FOXD3 antibody (R1107-4) at 1/5,000 dilution. Lane 1: NCCIT (Human teratoma cell) cell lysate Lane 2: HEK-293 (Human embryonic kidney cell) cell lysate Lane 3: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 60 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1107-4, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 48 kDa Observed band size: 48 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.