CD73 Rabbit Polyclonal Antibody
cat.: R1107-6
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 63kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human CD73 aa 231-280 / 574.
Positive control: Hela, A549, Jurkat, HT-29, HepG2.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IF-Cell
  FC
1:1,000-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P21589 Human | Q61503 Mouse
Alternative names: 5' NT 5' nucleotidase (CD73) 5' nucleotidase precursor 5' nucleotidase, ecto 5' nucleotidase, ecto (CD73) 5'-NT 5'-nucleotidase 5NTD_HUMAN CD73 CD73 antigen E5NT Ecto 5' nucleotidase Ecto-5'-nucleotidase eN eNT NT NT5 NT5E NTE Purine 5 Prime Nucleotidase
Images
R1107-6_1.jpg Fig1: Western blot analysis of CD73 on different cell lysates using anti-CD73 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: HT-29
R1107-6_2.jpg Fig2: ICC staining CD73 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
R1107-6_3.jpg Fig3: ICC staining CD73 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
R1107-6_4.jpg Fig4: Flow cytometric analysis of Jurkat cells with CD73 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.