CD73 Rabbit Polyclonal Antibody
cat.: R1107-6
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 63kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD73 aa 231-280 / 574. |
| Positive control: | Hela, A549, Jurkat, HT-29, HepG2. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P21589 Human | Q61503 Mouse |
| Alternative names: | 5' NT 5' nucleotidase (CD73) 5' nucleotidase precursor 5' nucleotidase, ecto 5' nucleotidase, ecto (CD73) 5'-NT 5'-nucleotidase 5NTD_HUMAN CD73 CD73 antigen E5NT Ecto 5' nucleotidase Ecto-5'-nucleotidase eN eNT NT NT5 NT5E NTE Purine 5 Prime Nucleotidase |
Images
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Fig1:
Western blot analysis of CD73 on different cell lysates using anti-CD73 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: HT-29 |
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Fig2: ICC staining CD73 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining CD73 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig4:
Flow cytometric analysis of Jurkat cells with CD73 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.