Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 18 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide corresponding to of Human THY-1 aa 1-50 / 161. |
Positive control: | U-87 MG cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate. |
Subcellular location: | Cell membrane |
Recommended Dilutions:
WB |
1:500-1:1,000 |
Uniprot #: | SwissProt: P04216 Human | P01831 Mouse | P01830 Rat |
Alternative names: | CD7 CD90 CD90 antigen CDw90 FLJ33325 MGC128895 T25 Theta antigen Thy 1 Thy 1 cell surface antigen Thy 1 membrane glycoprotein Thy 1 T cell antigen Thy 1.2 Thy-1 antigen Thy-1 membrane glycoprotein Thy1 Thy1 antigen Thy1 T cell antigen Thy1.1 Thy1.2 THY1_HUMAN Thymus cell antigen 1, theta |
Fig1:
Western blot analysis of CD90 / THY1 on different lysates with Rabbit anti-CD90 / THY1 antibody (R1107-9) at 1/1,000 dilution. Lane 1: U-87 MG cell lysate (15 µg/Lane) Lane 2: PC-12 cell lysate (15 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Lane 4: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 18 kDa Observed band size: 25 kDa Exposure time: 17 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1107-9) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |