NANOG Rabbit Polyclonal Antibody
cat.: R1108-2
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse NANOG aa 56-100. |
| Positive control: | NCCIT cell lysate, JAR cell lysate. |
| Subcellular location: | Nucleus |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q9H9S0 Human | Q80Z64 Mouse |
| Alternative names: | Embryonic stem cell specific homeobox protein (Nanog) ENK FLJ12581 hNanog Homeobox protein NANOG Homeobox transcription factor Nanog homeobox transcription factor Nanog-delta 48 NANOG Nanog homeobox NANOG_HUMAN |
Images
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Fig1:
Western blot analysis of NANOG on different lysates with Rabbit anti-NANOG antibody (R1108-2) at 1/500 dilution. Lane 1: NCCIT cell lysate Lane 2: JAR cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 35 kDa Observed band size: 37 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1108-2) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.