NCAM1 Rabbit Polyclonal Antibody
cat.: R1204-1
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 95 kDa
Isotype: IgG
Immunogen: Synthetic peptide corresponding to Mouse NCAM1 aa 101-150 / 1,115 mouse.
Positive control: SH-SY5Y cell lysate, NCCIT cell lysate, F9 cell lysate, C6 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, SH-SY5Y, N2A.
Subcellular location: Cell membrane, secreted
Recommended Dilutions:
  WB
  IF-Cell

1:1,000
1:50
Uniprot #: SwissProt: P13591 Human | P13595 Mouse | P13596 Rat
Alternative names: antigen recognized by monoclonal 5.1H11 CD56 Cell adhesion molecule, neural, 1 MSK39 N-CAM 140 N-CAM-1 NCAM NCAM-1 NCAM1 NCAM1_HUMAN Neural cell adhesion molecule 1 Neural cell adhesion molecule neural cell adhesion molecule, NCAM
Images
R1204-1_1.jpg Fig1: Western blot analysis of NCAM1 on different lysates with Rabbit anti-NCAM1 antibody (R1204-1) at 1/1,000 dilution.

Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: NCCIT cell lysate (20 µg/Lane)
Lane 3: F9 cell lysate (20 µg/Lane)
Lane 4: C6 cell lysate (20 µg/Lane)
Lane 5: Mouse brain tissue lysate (40 µg/Lane)
Lane 6: Rat brain tissue lysate (40 µg/Lane)

Predicted band size: 95 kDa
Observed band size: 120-180 kDa

Exposure time: 10 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1204-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
R1204-1_2.png Fig2: Immunocytochemistry analysis of SH-SY5Y cells labeling NCAM1 with Rabbit anti-NCAM1 antibody (R1204-1) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NCAM1 antibody (R1204-1) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
R1204-1_3.png Fig3: Immunocytochemistry analysis of N2A cells labeling NCAM1 with Rabbit anti-NCAM1 antibody (R1204-1) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NCAM1 antibody (R1204-1) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.