Geminin Rabbit Polyclonal Antibody
cat.: R1204-2
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 24 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Geminin aa 1-50 / 209.
Positive control: Hela cell lysates, NCCIT, F9, HepG2.
Subcellular location: Nucleus, cytoplasm
Recommended Dilutions:
  WB
  IF-Cell
  FC

1:500
1:50
1:50-1:100
Uniprot #: SwissProt: O75496 Human | O88513 Mouse
Alternative names: DNA replication inhibitor Gem GEMI_HUMAN Geminin Geminin DNA replication inhibitor GMNN OTTHUMP00000039393 RP3 369A17.3
Images
R1204-2_1.jpg Fig1: Western blot analysis of Geminin on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1204-2, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
R1204-2_2.jpg Fig2: ICC staining of Geminin in NCCIT cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1204-2, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
R1204-2_3.jpg Fig3: ICC staining of Geminin in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1204-2, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
R1204-2_4.jpg Fig4: Flow cytometric analysis of Geminin was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (R1204-2, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.