Geminin Rabbit Polyclonal Antibody
cat.: R1204-2
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 24 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Geminin aa 1-50 / 209. |
| Positive control: | Hela cell lysates, NCCIT, F9, HepG2. |
| Subcellular location: | Nucleus, cytoplasm |
| Recommended Dilutions:
WB IF-Cell FC |
1:500 1:50 1:50-1:100 |
| Uniprot #: | SwissProt: O75496 Human | O88513 Mouse |
| Alternative names: | DNA replication inhibitor Gem GEMI_HUMAN Geminin Geminin DNA replication inhibitor GMNN OTTHUMP00000039393 RP3 369A17.3 |
Images
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Fig1:
Western blot analysis of Geminin on different lysates with Rabbit anti-Geminin antibody (R1204-2) at 1/1,000 dilution. Lane 1: NCCIT Lane 2: HeLa Lane 2: Hep G2 Lysates/proteins at 20 µg/Lane. Exposure time: 6 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1204-2, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 24 kDa Observed band size: 30 kDa |
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Fig2: ICC staining of Geminin in NCCIT cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1204-2, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of Geminin in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1204-2, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: Flow cytometric analysis of Geminin was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (R1204-2, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.