PRMT5 Rabbit Polyclonal Antibody
cat.: R1204-3
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 73kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human Prtm5. |
| Positive control: | HepG2, NCCIT. |
| Subcellular location: | Cytoplasm, nucleus |
| Recommended Dilutions:
WB IF-Cell |
1:500 1:50 |
| Uniprot #: | SwissProt: O14744 Human | Q8CIG8 Mouse |
| Alternative names: | 72 kDa ICln binding protein 72 kDa ICln-binding protein ANM5_HUMAN Histone synthetic lethal 7, S. cerevisiae, homolog of Histone-arginine N-methyltransferase PRMT5 HMT1 hnRNP methyltransferase like 5 HOMOLOG OF; SKB1 HRMT1L5 IBP72 Jak-binding protein 1 JBP 1 JBP1 PRMT 5 PRMT5 Protein arginine methyltransferase 5 Protein arginine N methyltransferase 5 Protein arginine N methyltransferase 5 N terminally processed Protein arginine N-methyltransferase 5 S. POMBE S. POMBE HOMOLOG OF; SKB1 SHK1 KINASE BINDING PROTEIN 1 Shk1 kinase binding protein 1 homolog Shk1 kinase-binding protein 1 homolog Shk1 kinase/binding protein 1, S. pombe, homolog of SKB 1 SKB1 SKB1 homolog SKB1: SKB1 homolog (S. pombe) SKB1Hs |
Images
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Fig1: Western blot analysis of PRMT5 on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1204-3, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2: ICC staining of PSMA3 in NCCIT cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1204-3, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.