HRP Conjugated 6X His tag Rabbit Polyclonal Antibody
cat.: R1207-3
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide immune sequence is HHHHHHHHC. |
| Positive control: | C-terminal or N-terminal His-tagged recombinant protein |
| Recommended Dilutions:
WB |
1:2,000-1:5,000 |
| Alternative names: | 6 His epitope tag Hexa His tag HHHHHH epitope tag HHHHHH tag His tag Polyhistidine Tag |
Images
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Fig1:
Western blot analysis of 6X His tag on C-terminal His-tagged recombinant protein lysates with Rabbit anti-6X His tag antibody (R1207-3) at 1/10,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1207-3, 1/10,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 30 kDa Observed band size: 30 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.