Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Species independent |
Applications: | WB |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human Myc aa 410-420. |
Subcellular location: | Cell nuclear |
Recommended Dilutions:
WB |
1:500 |
Uniprot #: | SwissProt: P01106 Human |
Alternative names: | avian myelocytomatosis viral oncogene homolog bHLHe39 c-Myc class E basic helix-loop-helix protein 39 MRTL MYC Myc Epitope Tag myc proto-oncogene protein myc-related translation/localization regulatory factor oncogene c-Myc proto-oncogene c-Myc protooncogene homologous to myelocytomatosis virus transcription factor p64 v-myc avian myelocytomatosis viral oncogene homolog v-myc myelocytomatosis viral oncogene homolog (avian) |
Fig1:
Western blot analysis of Myc tag on different lysates with Rabbit anti-Myc tag antibody (R1208-2) at 1/500 dilution. Lane 1: C-terminal myc tagged protein Lane 2: N-terminal myc tagged protein Lysates/proteins at 50 ng/Lane. Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1208-2) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. |
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Fig2:
Advantages of using mAbs 1. High specificity Due to their recognition of only one epitope, mAbs are best known for their high specificity. Producing highly specific monoclonal antibodies supplies researchers with the benefits of improved target identification and less background signal. 2. Batch-to-batch reproducibility Since a monoclonal antibody is made by cloning a unique while blood cell. All subsequent products developed from this antibody trace back to its original, unique parent cell. This makes for great batch-to-batch reproducibility. 3. Guaranteed long term supply Hybridoma serves as a continuous source of monoclonal antibody. |