GAPDH Rabbit Polyclonal Antibody
cat.: R1208-3
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Magnaporthe oryzae, Zebrafish
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 36 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal human GAPDH.
Subcellular location: Cytoplasm, Nucleus
Recommended Dilutions:
  WB

1:2,000
Alternative names: 38 kDa BFA-dependent ADP-ribosylation substrate aging associated gene 9 protein Aging-associated gene 9 protein BARS-38 cb609 EC 1.2.1.12 Epididymis secretory sperm binding protein Li 162eP G3P_HUMAN G3PD G3PDH GAPD GAPDH Glyceraldehyde 3 phosphate dehydrogenase Glyceraldehyde-3-phosphate dehydrogenase HEL-S-162eP KNC-NDS6 MGC102544 MGC102546 MGC103190 MGC103191 MGC105239 MGC127711 MGC88685 OCAS, p38 component OCT1 coactivator in S phase, 38-KD component peptidyl cysteine S nitrosylase GAPDH Peptidyl-cysteine S-nitrosylase GAPDH wu:fb33a10
Images
R1208-3_1.jpg Fig1: Western blot analysis of GAPDH on HeLa cell lysate with Rabbit anti-GAPDH antibody (R1208-3) at 1/10,000 dilution.


Lysates/proteins at 15 µg/Lane.
Exposure time: 1 minutes; ECL: K1801


Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: R1208-3, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 36.1 kDa
Observed band size: 36 kDa
R1208-3_2.jpg Fig2: Western blot analysis of GAPDH on Magnaporthe oryzae with Rabbit anti-GAPDH antibody (R1208-3).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.