KAP1 Rabbit Polyclonal Antibody
cat.: R1210-2
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 89 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human TRIM28 aa 415-459. |
| Positive control: | Hela, NCCIT, F9, PC-3M, MCF-7, A431, human liver cancer tissue, human spleen tissue, human breast cancer tissue, human kidney tissue, mouse colon tissue, mouse kidney tissue. |
| Subcellular location: | Nucleus |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q13263 Human | Q62318 Mouse |
| Alternative names: | E3 SUMO protein ligase TRIM28 E3 SUMO-protein ligase TRIM28 FLJ29029 KAP 1 KAP-1 KRAB associated protein 1 KRAB interacting protein 1 KRAB-associated protein 1 KRAB-interacting protein 1 KRIP 1 KRIP-1 KRIP1 Nuclear corepressor KAP 1 Nuclear corepressor KAP-1 RING finger protein 96 RNF96 TF1B TIF1 beta TIF1-beta TIF1B TIF1B_HUMAN Transcription intermediary factor 1 beta Transcription intermediary factor 1-beta Trim28 Tripartite motif containing 28 tripartite motif containing protein 28 Tripartite motif-containing protein 28 |
Images
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Fig1: ICC staining TRIM28 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TRIM28 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TRIM28 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-TRIM28 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TRIM28 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-TRIM28 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TRIM28 antibody. Counter stained with hematoxylin. |
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Fig8:
Western blot analysis of KAP1 on different lysates with Rabbit anti-KAP1 antibody (R1210-2) at 1/1,000 dilution. Lane 1: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1210-2, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 89 kDa Observed band size: 100 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.