Transferrin Rabbit Polyclonal Antibody
cat.: R1212-1
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 77 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Transferrin aa 20-698. |
| Positive control: | Isolated human transferrin, human serum lysates, HepG2. |
| Recommended Dilutions:
WB IF-Cell |
1:2,000-1:5,000 1:250 |
| Uniprot #: | SwissProt: P02787 Human |
| Alternative names: | Apotransferrin Beta 1 metal binding globulin Beta-1 metal-binding globulin DKFZp781D0156 PRO1400 PRO1557 PRO2086 Serotransferrin Serotransferrin precursor Siderophilin TF TFQTL1 Transferin Transferrin TRFE_HUMAN |
Images
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Fig1: Western blot analysis on isolated human transferrin (1) and human serum (2) lysates using anti-transferrin rabbit polyclonal antibody. |
|
Fig2:
Immunocytochemistry analysis of HepG2 cells labeling Transferrin with Rabbit anti-Transferrin antibody (R1212-1) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Transferrin antibody (R1212-1) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.