CD24 Rabbit Polyclonal Antibody
cat.: R1303-1
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 8 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD24 aa 1-50 / 80. |
| Positive control: | MCF7 cell lysates, human colon carcinoma tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P25063 Human |
| Alternative names: | CD 24 CD24 CD24 antigen (small cell lung carcinoma cluster 4 antigen) CD24 antigen CD24 molecule CD24_HUMAN CD24A FLJ22950 FLJ43543 GPI linked surface mucin Heat stable antigen HSA MGC75043 Nectadrin Signal transducer CD24 Small cell lung carcinoma cluster 4 antigen |
Images
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Fig1:
Western blot analysis of CD24 on MCF7 cell lysates with Rabbit anti-CD24 antibody (R1303-1) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 8 kDa Observed band size: 45 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1303-1) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-CD24 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1303-1, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.