Stella Rabbit Polyclonal Antibody
cat.: R1306-1
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Zebrafish |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 18 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse Stella aa 101-150 / 150 (ID:Q8QZY). |
| Positive control: | HeLa cell lysate, HEK-293 cell lysate, F9 cell lysate, NCCIT cell lysate, mouse teratoma tissue. |
| Subcellular location: | Cell nucleus, cytoplasm |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:100-1:200 |
| Uniprot #: | SwissProt: Q6W0C5 Human Entrez Gene: 73708 Mouse |
| Alternative names: | Compaction associated protein 1 Developmental pluripotency associated 3 Developmental pluripotency associated protein 3 Developmental pluripotency-associated protein 3 Dppa3 DPPA3_HUMAN PGC7 Stella related protein Stella-related protein |
Images
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Fig1:
Western blot analysis of Stella on different lysates with Rabbit anti-Stella antibody (R1306-1) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: F9 cell lysate Lane 4: NCCIT cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 18 kDa Observed band size: 25 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1306-1) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded mouse teratoma tissue using anti-Stella rabbit polyclonal antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.