PCNA Rabbit Polyclonal Antibody
cat.: R1306-5
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 29 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human PCNA aa 21-82.
Positive control: HeLa cell lysate, Raji cell lysate, HepG2 cell lysate, L929 cell lysate, SK-Br-3 cell lysate, F9 cell lysate, PC-12 cell lysate, Hela, human colon tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P

1:1,000
1:100
1:200
Uniprot #: SwissProt: P12004 Human | P17918 Mouse | P04961 Rat
Alternative names: ATLD2 cb16 Cyclin DNA polymerase delta auxiliary protein etID36690.10 fa28e03 fb36g03 HGCN8729 MGC8367 Mutagen-sensitive 209 protein OTTHUMP00000030189 OTTHUMP00000030190 PCNA Pcna/cyclin PCNA_HUMAN PCNAR Polymerase delta accessory protein Proliferating cell nuclear antigen wu:fa28e03 wu:fb36g03
Images
R1306-5_1.jpg Fig1: Western blot analysis of PCNA on different lysates with Rabbit anti-PCNA antibody (R1306-5) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: Raji cell lysate
Lane 3: HepG2 cell lysate
Lane 4: L929 cell lysate
Lane 5: SK-Br-3 cell lysate
Lane 6: F9 cell lysate
Lane 7: PC-12 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 29 kDa
Observed band size: 29 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1306-5) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
R1306-5_2.jpg Fig2: Immunofluorescent staining of Hela cells using anti- PCNA rabbit polyclonal antibody.
R1306-5_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PCNA rabbit polyclonal antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.