VDAC1 Rabbit Polyclonal Antibody
cat.: R1307-1
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Zebrafish
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 31 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human VDAC1 aa 188-231.
Positive control: HeLa cell lysate, Jurkat cell lysate, A431 cell lysate, Raji cell lysate, HepG2 cell lysate, SW480 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate, Hela, human skeletal mucle tissue, mouse skeletal mucle tissue, rat skeletal mucle tissue.
Subcellular location: Mitochondrial membrane, cell membrane
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:1,000-1:2,000
1:200
1:500
Uniprot #: SwissProt: P21796 Human
Alternative names: N2441 OMP2 POR1 hVDAC1 MGC111064 Mitochondrial Porin Outer mitochondrial membrane protein porin 1 Plasmalemmal porin Porin 31HL Porin 31HM VDAC VDAC-1 Vdac1 VDAC1_HUMAN Voltage dependent anion channel 1 Voltage dependent anion selective channel protein 1 Voltage-dependent anion-selective channel protein 1 YNL055C YNL2441C
Images
R1307-1_1.jpg Fig1: Western blot analysis of VDAC1 on different lysates with Rabbit anti-VDAC1 antibody (R1307-1) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: A431 cell lysate
Lane 4: Raji cell lysate
Lane 5: HepG2 cell lysate
Lane 6: SW480 cell lysate
Lane 7: A549 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 31 kDa
Observed band size: 31 kDa

Exposure time: 1 minute 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1307-1) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
R1307-1_2.jpg Fig2: Western blot analysis of VDAC1 on different lysates with Rabbit anti-VDAC1 antibody (R1307-1) at 1/2,000 dilution.

Lane 1: NIH/3T3 cell lysate (20 µg/Lane)
Lane 2: C2C12 cell lysate (20 µg/Lane)
Lane 3: C6 cell lysate (20 µg/Lane)
Lane 4: Mouse skeletal muscle tissue lysate (40 µg/Lane)
Lane 5: Rat skeletal muscle tissue lysate (40 µg/Lane)

Predicted band size: 31 kDa
Observed band size: 31 kDa

Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1307-1) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
R1307-1_3.jpg Fig3: Western blot analysis of VDAC1 on different lysates with Rabbit anti-VDAC1 antibody (R1307-1) at 1/1,000 dilution.

Lane 1: HEK293-si NT cell lysate (10 µg/Lane)
Lane 2: HEK293-si VDAC1#1(no heat) cell lysate (10 µg/Lane)
Lane 3: HEK293-si VDAC1#2(no heat) cell lysate (10 µg/Lane)

Notice: no heat means the lysate is not boiled.

Predicted band size: 31 kDa
Observed band size: 31 kDa

Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.

R1307-1 was shown to specifically react with VDAC1 in HEK293-si NT cells. No band were observed when HEK293-si VDAC1 samples were tested. HEK293-si NT and HEK293-si VDAC1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (R1307-1, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at 4℃ overnight. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
R1307-1_4.jpg Fig4: ICC staining VDAC1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
R1307-1_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human skeletal mucle tissue with Rabbit anti-VDAC1 antibody (R1307-1) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1307-1) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
R1307-1_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse skeletal mucle tissue with Rabbit anti-VDAC1 antibody (R1307-1) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1307-1) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
R1307-1_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat skeletal mucle tissue with Rabbit anti-VDAC1 antibody (R1307-1) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1307-1) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.