VDAC1 Rabbit Polyclonal Antibody
cat.: R1307-1
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Zebrafish |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human VDAC1 aa 188-231. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, A431 cell lysate, Raji cell lysate, HepG2 cell lysate, SW480 cell lysate, A549 cell lysate, human brain tissue, Hela. |
| Subcellular location: | Mitochondrial membrane, cell membrane |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000 1:200 1:200 |
| Uniprot #: | SwissProt: P21796 Human |
| Alternative names: | N2441 OMP2 POR1 hVDAC1 MGC111064 Mitochondrial Porin Outer mitochondrial membrane protein porin 1 Plasmalemmal porin Porin 31HL Porin 31HM VDAC VDAC-1 Vdac1 VDAC1_HUMAN Voltage dependent anion channel 1 Voltage dependent anion selective channel protein 1 Voltage-dependent anion-selective channel protein 1 YNL055C YNL2441C |
Images
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Fig1:
Western blot analysis of VDAC1 on different lysates with Rabbit anti-VDAC1 antibody (R1307-1) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: A431 cell lysate Lane 4: Raji cell lysate Lane 5: HepG2 cell lysate Lane 6: SW480 cell lysate Lane 7: A549 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1307-1) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-VDAC1 rabbit polyclonal antibody. |
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Fig3:
Western blot analysis of VDAC1 on different lysates with Rabbit anti-VDAC1 antibody (R1307-1) at 1/1,000 dilution. Lane 1: HEK293-si NT cell lysate Lane 2: HEK293-si VDAC1#1(no heat) cell lysate Lane 3: HEK293-si VDAC1#2(no heat) cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. R1307-1 was shown to specifically react with VDAC1 in HEK293-si NT cells. No band were observed when HEK293-si VDAC1 samples were tested. HEK293-si NT and HEK293-si VDAC1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (R1307-1, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at 4℃ overnight. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig4: ICC staining VDAC1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.