Nucleolin Rabbit Polyclonal Antibody
cat.: R1307-2
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 77 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Nucleolin aa 201-250 / 710. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, HEK-293 cell lysate, A431 cell lysate, HepG2 cell lysate, A549 cell lysate, mouse embryonic stem. |
| Subcellular location: | Nucleus, nucleolus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100-1:200 |
| Uniprot #: | SwissProt: P19338 Human |
| Alternative names: | C23 FLJ45706 MS1116 NCL Nucl NUCL_HUMAN Nucleolin Protein C23 |
Images
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Fig1:
Western blot analysis of Nucleolin on different lysates with Rabbit anti-Nucleolin antibody (R1307-2) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: HEK-293 cell lysate Lane 4: A431 cell lysate Lane 5: HepG2 cell lysate Lane 6: A549 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 77 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1307-2) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2: ICC staining nucleolin in mouse embryonic stem cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.