IRX2 Rabbit Polyclonal Antibody
cat.: R1307-5
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 49 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to Human IRX2 aa 422-471 / 471. |
| Positive control: | Hela cell lysate, HepG2 cell lysate, SiHa cell lysate, Hela. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q9BZI1 Human |
| Alternative names: | Homeodomain protein IRXA2 Iroquois homeobox 2 Iroquois homeobox protein 2 Iroquois-class homeodomain protein irx-2 IRX 2 irx2 IRX2_HUMAN IrxA2 |
Images
|
Fig1:
Western blot analysis of IRX2 on different lysates with Rabbit anti-IRX2 antibody (R1307-5) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lane 3: SiHa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 49 kDa Observed band size: 49 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1307-5) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunofluorescent staining of Hela cells using anti- IRX2rabbit polyclonal antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.