VMAT1 Rabbit Polyclonal Antibody
cat.: R1307-7
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human VMAT1 aa 91-140 / 525. |
| Positive control: | A549 cell lysate, SH-SY5Y cell lysate, PC-12 cell lysate, PANC-1 cell lysate, A549. |
| Subcellular location: | Cytoplasmic vesicle membrane; Endoplasmic reticulum membrane |
| Recommended Dilutions:
WB IF-Cell |
1:500 1:100 |
| Uniprot #: | SwissProt: P54219 Human | Q01818 Rat |
| Alternative names: | 4832416I10Rik Adrenal chromaffin granule amine transporter; CGAT CGAT Chromaffin granule amine transporter MGC28683 MGC37299 SLC18A1 Solute carrier family 18 (vesicular monoamine), member 1 Solute carrier family 18 member 1 VAT 1 VAT1 Vesicular amine transporter 1 VMAT 1 VMAT1_HUMAN |
Images
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Fig1:
Western blot analysis of VMAT1 on different lysates with Rabbit anti-VMAT1 antibody (R1307-7) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: PC-12 cell lysate Lane 4: PANC-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 56 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1307-7) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunofluorescent staining of A549 cells using anti-VMAT1 rabbit polyclonal antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.