KLF4 Rabbit Polyclonal Antibody
cat.: R1308-1
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human KLF4 aa 406-458. |
| Positive control: | F9 cell lysate, Rat liver tissue lysate, 293 cell lysates, human colon tissue, human skin tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:200-1:1,500 1:100 |
| Uniprot #: | SwissProt: O43474 Human | Q60793 Mouse Entrez Gene: 114505 Rat |
| Alternative names: | Endothelial Kruppel like zinc finger protein Epithelial zinc finger protein EZF EZF GKLF gut Kruppel-like factor Gut-enriched krueppel-like factor KLF KLF4 KLF4_HUMAN Krueppel-like factor 4 Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor) Kruppel like factor 4 (gut) |
Images
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Fig1:
Western blot analysis of KLF4 on different lysates with Rabbit anti-KLF4 antibody (R1308-1) at 1/1,000 dilution. Lane 1: F9 cell lysate (20 µg/Lane) Lane 2: Rat liver tissue lysate (30 µg/Lane) Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 27 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1308-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Western blot analysis on 293 (1) and F9 (2) cell lysates using anti-KLF4 rabbit polyclonal antibodies. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-KLF4 antibody (R1308-1) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1308-1) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-KLF4 antibody (R1308-1) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1308-1) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.