BCL2L1 Rabbit Polyclonal Antibody
cat.: R1308-16
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human BCL2L1. |
| Positive control: | HeLa cell lysate, A549 cell lysate, Jurkat cell lysate, PC-12 cell lysate, human kidney tissue, mouse kidney tissue. |
| Subcellular location: | Mitochondrion membrane, nucleus membrane, cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Nucleus, Synapse. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:50 |
| Uniprot #: | SwissProt: Q07817 Human | Q64373 Mouse | P53563 Rat |
| Alternative names: | Apoptosis regulator Bcl X Apoptosis regulator Bcl-X Apoptosis regulator BclX B cell lymphoma 2 like B2CL1_HUMAN Bcl 2 like 1 protein Bcl X Bcl xL BCL XL/S Bcl xS Bcl-2-like protein 1 Bcl2 Like 1 Bcl2 related gene Bcl2-L-1 BCL2L Bcl2l1 BCLX BclXL BclXs DKFZp781P2092 PPP1R52 Protein phosphatase 1 regulatory subunit 52 |
Images
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Fig1:
Western blot analysis of BCL2L1 on different lysates with Rabbit anti-BCL2L1 antibody (R1308-16) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: PC-12 cell lysate (20 µg/Lane) Predicted band size: 26 kDa Observed band size: 30 kDa Exposure time: 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1308-16) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-BCL2L1 antibody (R1308-16) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1308-16) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-BCL2L1 antibody (R1308-16) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1308-16) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.