BCL2L1 Rabbit Polyclonal Antibody
cat.: R1308-16
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human BCL2L1. |
| Positive control: | Hela, A431 cell lysate, K562 cell lysate, human kidney tissue, mouse kidney tissue. |
| Subcellular location: | Mitochondrion membrane, nucleus membrane, cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Nucleus, Synapse. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1,000 1:100-1:200 1:200 |
| Uniprot #: | SwissProt: Q07817 Human |
| Alternative names: | Apoptosis regulator Bcl X Apoptosis regulator Bcl-X Apoptosis regulator BclX B cell lymphoma 2 like B2CL1_HUMAN Bcl 2 like 1 protein Bcl X Bcl xL BCL XL/S Bcl xS Bcl-2-like protein 1 Bcl2 Like 1 Bcl2 related gene Bcl2-L-1 BCL2L Bcl2l1 BCLX BclXL BclXs DKFZp781P2092 PPP1R52 Protein phosphatase 1 regulatory subunit 52 |
Images
|
Fig1:
Western blot analysis on A431 and K562 cell lysates using anti-BCL2L1rabbit polyclonal antibodies. |
|
Fig2:
Immunofluorescent staining of Hela cells using anti- BCL2L1 rabbit polyclonal antibody. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-BCL2L1 antibody (R1308-16) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1308-16) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-BCL2L1 antibody (R1308-16) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1308-16) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.