ESRRB Rabbit Polyclonal Antibody
cat.: R1308-2
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ESRRB aa 168-212. |
| Positive control: | 293T cell lysate, NCCIT cell lysate, Caco-2 cell lysate. |
| Subcellular location: | Nucleus, Cytoplasm, Chromosome. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:2,000 1:200 1:100-1:200 |
| Uniprot #: | SwissProt: O95718 Human |
| Alternative names: | Err 2 ERR b ERR B2 ERR beta 2 ERR beta ERR beta-2 ERR-beta Err2 ERR2_HUMAN ERRB 2 ERRb ERRB2 ERRbeta 2 ERRbeta ESR L2 ESRL 2 ESRL2 Esrrb Estrogen receptor like 2 Estrogen receptor related 2 Estrogen receptor-like 2 Estrogen-related receptor beta Estrrb Nr3b2 Nuclear receptor ERRB2 Nuclear receptor subfamily 3 group B member 2 Orphan nuclear receptor Steroid hormone receptor ERR 2 Steroid hormone receptor ERR2 |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti- ESRRB rabbit polyclonal antibody. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-ESRRB rabbit polyclonal antibody. |
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Fig3: ICC staining ESRRB in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). |
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Fig4:
Western blot analysis of ESRRB on different lysates with Rabbit anti-ESRRB antibody (R1308-2) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: NCCIT cell lysate Lane 3: Caco-2 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1308-2, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 48 kDa Observed band size: 56 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.