Vimentin Rabbit Polyclonal Antibody
cat.: R1308-6
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within residues of Human Vimentin aa 417-466 / 466. |
| Positive control: | Hela cell lysate, A549 cell lysate, 293T cell lysate, Hela, human breast carcinoma tissue, Wild-type Hela whole cell lysate. |
| Subcellular location: | Cytoplasm, Cell membrane, Cytoskeleton, Intermediate filament, Membrane, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:2,000 1:100-1:200 1:200 |
| Uniprot #: | SwissProt: P08670 Human |
| Alternative names: | CTRCT30 Epididymis luminal protein 113 FLJ36605 HEL113 VIM VIME_HUMAN Vimentin |
Images
|
Fig1:
Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (R1308-6) at 1/2,000 dilution. Lane 1: Hela cell lysate Lane 2: A549 cell lysate Lane 3: 293T cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 54 kDa Observed band size: 56 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1308-6) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
All lanes: Western blot analysis of Vimentin with anti-Vimentin antibody (R1308-6) at 1/500 dilution. Lane 1: Wild-type Hela whole cell lysate (10 µg). Lane 2: Vimentin knockout Hela whole cell lysate (10 µg). R1308-6 was shown to specifically react with Vimentin in wild-type Hela cells. No band was observed when Vimentin knockout sample was tested. Wild-type and Vimentin knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (R1308-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3: Immunofluorescent staining of Hela cells using anti- vimentin rabbit polyclonal antibody. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-vimentin rabbit polyclonal antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.