p53 Rabbit Polyclonal Antibody
cat.: R1308-7
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human P53. |
| Positive control: | MDA-MB-231 cell lysate, HEK-293 cell lysate, MDA-MB-468 cell lysate, A431. |
| Subcellular location: | Cytoplasm, Nucleus, Cytoskeleton, Endoplasmic reticulum, Mitochondrion. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: P04637 Human |
| Alternative names: | Antigen NY-CO-13 BCC7 Cellular tumor antigen p53 FLJ92943 LFS1 Mutant tumor protein 53 p53 p53 tumor suppressor P53_HUMAN Phosphoprotein p53 Tp53 Transformation related protein 53 TRP53 Tumor protein 53 Tumor protein p53 Tumor suppressor p53 |
Images
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Fig1:
Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (R1308-7) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: HEK-293 cell lysate Lane 3: MDA-MB-468 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1308-7) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (R1308-7) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-p53 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1308-7) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3: Immunofluorescent staining of A431 cells using anti- p53 rabbit polyclonal antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.