GPR132 Rabbit Polyclonal Antibody
cat.: R1308-8
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human GPR132 aa 1-50 / 380. |
| Positive control: | HeLa cell lysate, 293T cell lysate, Human brain tissue lysate, Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate, A172. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:2,000-1:5,000 1:100 |
| Uniprot #: | SwissProt: Q9UNW8 Human | Q9Z282 Mouse |
| Alternative names: | G Protein Coupled Receptor G2A G2 accumulation protein G2A GP132_HUMAN GPR132 Probable G-protein coupled receptor 132 |
Images
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Fig1:
Western blot analysis of GPR132 on different lysates with Rabbit anti-GPR132 antibody (R1308-8) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: Human brain tissue lysate (40 µg/Lane) Lane 4: Mouse skeletal muscle tissue lysate (40 µg/Lane) Lane 5: Rat skeletal muscle tissue lysate (40 µg/Lane) Predicted band size: 42 kDa Observed band size: 45 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1308-8) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunofluorescent staining of A172 cells using anti- GPR132 rabbit polyclonal antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.