GFAP Rabbit Polyclonal Antibody
cat.: R1308-9
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human GFAP. |
| Positive control: | Human brain tissue lysates, mouse brain tissue lysates, A172, human brain tissue, rat hippocampus tissue. |
| Subcellular location: | Cytoplasm, Intermediate filament. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:2,000 1:50-1:200 1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P14136 Human |
| Alternative names: | wu:fb34h11 ALXDRD cb345 etID36982.3 FLJ42474 FLJ45472 GFAP GFAP_HUMAN gfapl Glial fibrillary acidic protein Intermediate filament protein wu:fk42c12 xx:af506734 zgc:110485 |
Images
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Fig1: Western blot analysis on human brain tissue lysates using anti-GFAP rabbit polyclonal antibodies. |
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Fig2:
Western blot analysis of GFAP on mouse brain tissue lysates with Rabbit anti-GFAP antibody (R1308-9) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1308-9) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig3: Immunofluorescent staining of A172 cells using anti- GFAP rabbit polyclonal antibody. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human brain tissue using anti- GFAP rabbit polyclonal antibody. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-GFAP antibody (R1308-9) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1308-9) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.