HMGB1 Rabbit Polyclonal Antibody
cat.: R1309-12
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human HMGB1. |
| Positive control: | Wild-type Raw264.7 whole cell lysate, MCF-7 cell lysate, PC12 cell lysate, F9 cell lysate, Hela, mouse spleen tissue. |
| Subcellular location: | Nucleus, Cell membrane, Chromosome, Cytoplasm, Endoplasmic reticulum, Endosome, Membrane, Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000-1:20,000 1:200-1:500 1:200 1:100 |
| Uniprot #: | SwissProt: P09429 Human |
| Alternative names: | Amphoterin Chromosomal protein, nonhistone, HMG1 DKFZp686A04236 High mobility group 1 High mobility group box 1 High mobility group protein 1 High mobility group protein B1 high-mobility group (nonhistone chromosomal) protein 1 HMG-1 HMG1 HMG3 HMGB 1 HMGB1 HMGB1_HUMAN NONHISTONE CHROMOSOMAL PROTEIN HMG1 SBP 2 Sulfoglucuronyl carbohydrate binding protein |
Images
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Fig1:
Western blot analysis of HMGB1 on different lysates with Rabbit anti-HMGB1 antibody (R1309-12) at 1/20,000 dilution. Lane 1: MCF7 (Human breast cancer cell) cell lysate Lane 2: F9 (Mouse teratocarcinoma cell) cell lysate Lane 3: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1309-12, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 25 kDa Observed band size: 25 kDa |
|
Fig2: Immunofluorescent staining of Hela cells using anti- HMGB1 rabbit polyclonal antibody. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti- HMGB1 rabbit polyclonal antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.