HMGB1 Rabbit Polyclonal Antibody
cat.: R1309-12
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human HMGB1. |
| Positive control: | Wild-type Raw264.7 whole cell lysate, MCF-7 cell lysate, PC12 cell lysate, F9 cell lysate, Hela, mouse spleen tissue. |
| Subcellular location: | Nucleus, Cell membrane, Chromosome, Cytoplasm, Endoplasmic reticulum, Endosome, Membrane, Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000-1:2,000 1:200-1:500 1:200 1:100 |
| Uniprot #: | SwissProt: P09429 Human |
| Alternative names: | Amphoterin Chromosomal protein, nonhistone, HMG1 DKFZp686A04236 High mobility group 1 High mobility group box 1 High mobility group protein 1 High mobility group protein B1 high-mobility group (nonhistone chromosomal) protein 1 HMG-1 HMG1 HMG3 HMGB 1 HMGB1 HMGB1_HUMAN NONHISTONE CHROMOSOMAL PROTEIN HMG1 SBP 2 Sulfoglucuronyl carbohydrate binding protein |
Images
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Fig1:
All lanes: Western blot analysis of HMGB1 with anti-HMGB1 antibody (R1309-12) at 1/500 dilution. Lane 1: Wild-type Raw264.7 whole cell lysate. Lane 2: HMGB1 knockout Raw264.7 whole cell lysate. R1309-12 was shown to specifically react with HMGB1 in wild-type Raw264.7 cells. No band was observed when HMGB1 knockout sample was tested. Wild-type and HMGB1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-HMGB1 antibody (R1309-12, 1/500) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China). |
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Fig2: Western blot analysis on MCF-7 (1), PC12 (2) and F9 (3) cell lysates using anti-HMGB1 rabbit polyclonal antibodies. |
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Fig3: Immunofluorescent staining of Hela cells using anti- HMGB1 rabbit polyclonal antibody. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti- HMGB1 rabbit polyclonal antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.